|
KMID : 0043320170400121443
|
|
Archives of Pharmacal Research
2017 Volume.40 No. 12 p.1443 ~ p.1454
|
|
|
The signaling of protease-activated receptor-2 activating peptide-induced contraction in cat esophageal smooth muscle cells
|
|
Ha Hyun-Su
Lee Se-Eun
Lee Hyun-Seok
Kim Gil-Hyung
Yoon Chan-Jong
Han Jong-Soo
Lee Ji-Yun
Sohn Uy-Dong
|
|
|
Abstract
|
|
|
|
Protease-activated receptors (PARs) are a family of G protein-coupled receptors with a unique activation mechanism involving proteolytic cleavage of the extracellular N-terminal domain of the receptor. PAR2 has a contractile effect on esophageal smooth muscle. We investigate the signaling pathways of the PAR2-activating peptide (PAR2-AP) induced contraction in cat esophageal smooth muscle cells. The length of freshly isolated smooth muscle cells and permeabilized cells from feline esophagus were measured by scanning micrometry, and by confirming molecular basis via western blot analysis. The responses to PAR2-AP were initial and sustained contractions, depending on time. The maximum contraction of the initial phase occurred at 60 s. The PAR2-AP-induced contraction was mediated by Gαi1, Gαi3, and Gαq protein activation, leading to phospholipase-c (PLC) and myosin light chain kinase (MLCK) activation. 20 kDa myosin light chain (MLC20) was phosphorylated by PAR2-AP. Rho kinase-2 (ROCK-2), an activator of 17 kDa C-kinase potentiated Protein phosphatase-1 Inhibitor (CPI-17), was increased by PAR2 receptor activation. In conclusion, PAR2-AP produced an initial contraction mediated by Gαi1, Gαi3, and Gαq protein activation, resulting in PLC and MLCK activation. The sustained contraction by PAR2-AP was mediated by the Rho/Rho kinase-dependent pathway.
|
|
|
KEYWORD
|
|
|
PAR2-AP, Cat esophageal smooth muscle cells, Contraction, G proteins
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|